Keywords
PD-L1, PD-1, Immunotherapy, Lung Cancer, NSCLC, Image Analysis
Methods
To obtain H and DAB colour channels of the image the color deconvolution algorithm is used. Nuclei are detected on H channel and nuclei border pixels are evaluated one by one. Nuclei border pixels on DAB channel are analysed according to positive limit value and rate positive or negative.
Workflows
Step 1: View a PD-L1 stained lung cancer whole slide image at ViraPath.
Step 2: Outline tumor either manually or automatically using Virapath Tissue Segmentation algorithm.
Step 3: Select the PD-L1 analysis and calibrate the parameters.
Step 4: Run the analysis.
References
[1] Ishida, Y., Agata, Y., Shibahara, K., and Honjo, T. (1992). Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 11, 3887–3895.
[2] Sun, C., Mezzadra, R., & Schumacher, T. N. (2018). Regulation and Function of the PD-L1 Checkpoint. Immunity, 48(3), 434–452.
[3] Patel, S. P., & Kurzrock, R. (2015). PD-L1 Expression as a Predictive Biomarker in Cancer Immunotherapy. Molecular Cancer Therapeutics, 14(4), 847–856.