Keywords
Gene mutations, molecular pathology, fluorescent probes, image analysis
Methods
First, signal sizes and their locations are analyzed by using several filter algorithms. Second, detected cell nuclei are classified as positive, negative and artifact according to given probe type.
Quantitative output variables
1.Amplification for HER2 Gene
- Total green signal count
- Total red signal count
- Average HER2/CEP17
- HER2 copy count
- cep17 copy count
- ISH status
- Total positive ROI
- List of all dapi with signal counts
2. Amplification for other specific genes
- Total green signal count
- Total red signal count
- Total specific genes amplification
- Total negative ROI
- Positivity Index
- List of all dapi with signal counts
3.Deletion
- Total green signal count
- Total red signal count
- Total specific genes deletion
- Total negative ROI
- Positivity Index
- List of all dapi with signal counts
4.Break Apart
- Total positive ROI
- Total negative ROI
- Positivity Index
- List of all dapi with signal counts, break apart and fusion status
5.Fusion
- Total positive ROI
- Total negative ROI
- Positivity Index
- List of all dapi with signal numbers, break apart and fusion status
References
1] Ratan, Z. A., Zaman, S. B., Mehta, V., Haidere, M. F., Runa, N. J., & Akter, N. (2017). Application of Fluorescence In Situ Hybridization (FISH) Technique for the Detection of Genetic Aberration in Medical Science. Cureus